Institute for Automation and Applied Informatics

Robot and Computer Assisted Microscopy

In cooperation with biologists several robot microscopes have been developed to realize high throughput screening (HTS) with a large number of zebrafish eggs or embryos.

1. Screening of 96 micro well plates within a minute, fluorescence microscopy, videos and time-lapse recordings are also possible.

2. Optionally automated feature detection is possible e.g. to automatically detect the heart of non-fixed and non-anaesthetised zebrafish in 96 micro well plates. Videos of the heart region are recorded and the beat-to-beat intervals are identified automatically.

3. Sorting of zebrafish eggs or hatched larvae into 96 micro well plates can be done with our fish-sorter. It can also distinguish between fertilized and coagulated eggs, fluorescence or pigmentation patterns.

 

Robot and Computer Assisted Microscopy

 

 

  • M. Schutera, T. Dickmeis, M. Mione, R. Peravali, D. Marcato, M. Reischl, R. Mikut, C. Pylatiuk: Automated phenotype pattern recognition of zebrafish for high-throughput screening. Bioengineered. 7(4):261-5C, 2016. 

  • S. Nasrat, D. Marcato, S. Hirth, M. Reischl, C. Pylatiuk: Semi-automated Detection of Fractional Shortening in Zebrafish Embryo Heart Videos. Current Directions in Biomedical Engineering 2(1):233-236, 2016. 

  • D. Marcato, H. Breitwieser, P. Scheikl, R. Peravali, M. Reischl, C. Pylatiuk: Beispiele für den Einsatz von Automatisierungs-technik bei der Analyse biologischer Modellorganismen. at – Automatisierungstechnik, 64(11), 915-925, 2016.           

  • Spomer, W., et al.: High-Throughput Screening of Zebrafish Embryos Using Automated Heart Detection and Imaging. J. Lab. Autom. 17(6):435-442, 2012.
  • Pfriem, A., et al.: A modular, low-cost robot for zebrafish handling. Conf. Proc. IEEE EMBS, 980-983, 2012.
  • Pylatiuk, C. et al.: Automatic zebrafish heartbeat detection and analysis for zebrafish embryos. Zebrafish, 11(4):379-383, 2014.
  • Marcato, D. et al: An Automated and High-throughput Photomotor Response Platform for Chemical Screens. Conf. Proc. EMBS, 7728-7731, 2015